Interaction between immune modulators and inflammation factors considered as main underlying pathology of Non-alcoholic fatty liver disease (NAFLD). Cytokines of IL-1B,IL-18 and IL-33 and melatonin has a interaction that may play a major role in developing and progress NAFLD. We try to clear association between these cytokines and melatonin.

In this cross-sectional study adult patients with diagnosis of fatty liver disease by ultrasonography. All of them underwent Fibroscan evaluation. Subjects who met the inclusion and exclusion criteria for NAFLD were involved. A normal group also selected. The participants were categorized into the three following groups" 1) fibrosis> 9.1kp and steatosis>290 dbm) 2) fibrosis: 6-9.0 KP and steatosis 240-290, and 3) normal group with fibrosis< 6.0 KP and steatosis< 240 dbm. laboratory assessment and a questionnaire including demographic, anthropometric, laboratories and clinical data were completed for each of them.

A total 97 persons included. The mean age was 42.21± 11 years old. 59 patients (60.0%) were female. Cytokines levels have significant associated with liver enzymes. The melatonin levels as well as IL-1 family levels were increased by advancing fibrosis and steatosis. In univariate analysis we observed a significant association between these cytokines and advancing fibrosis ,sever steatosis and melatonin concentration. Furthermore ,in multiple linear regression model melatonin level has a significant association with levels of these cytokines

Melatonin may modulate the developing and progression of NAFLD via influence on IL-1B family .Therefore consider the therapeutic model that effect on this process may has great impact.