Background: Inflammatory bowel disease (IBD) is an idiopathic and chronic inflammation of the intestine that includes ulcerative colitis and Crohn's disease. In Asia and the Middle East, the highest incidence of Crohn's disease is 5 per 100,000 per year. Crohn's disease is a chronic inflammatory disease that affects different parts of the gastrointestinal tract from the mouth to the anus, but in most cases it affects the end of the small intestine.

Materials and Methods: In this clinical trial study, which was initially conducted as a pilot study, four patients with Crohn's disease were selected for this study. Consent. Ethanolic extract of Trachyspermum ammi was given twice a day for 3 months and Clinical symptoms and their calprotectin levels were measured.

Results: The first patient was a 39-year-old woman with an initial calprotectin level of 840 mg/kg, after taking the drug the calprotectin level was 10.72 mg/kg after the second month. The second patient was a 22-year-old man whose initial calprotectin level was 760 mg/kg and after the intervention his calprotectin level was 260 mg/kg. The third patient was a 33-year-old man with an initial calprotectin level of 2225 mg/kg. After taking the drug, the calprotectin level became 1270 mg/kg after the second month. The fourth patient was a 35-year-old woman with an initial calprotectin level of 535 mg/kg. After taking the drug, calprotectin levels rose to 100 mg/kg after the intervention.

Conclusion: It seems that Trachyspermum ammi plant is effective in reducing calprotectin levels in patients with Crohn's disease, but its effect and mechanism need further studies in the future. In this study, which was conducted for the first time in the world, by evaluating the patients by clinical condition, laboratory findings, colonoscopy and pathology, it was shown that this drug can be used in patients with Crohn's disease, resistant to Usual treatments (Mesalazine, Azathioprine, Cortone, etc.) and even biological drugs are effective and appropriate treatments ,also, it has no significant complications and has a much lower cost.