Irritable bowel syndrome (IBS) is a functional gastrointestinal disorder with recurrent abdominal pain and associated with defecation or change in bowel habits (constipation, diarrhea or a mix of constipation and diarrhea). All symptoms occur in the absence of metabolic or organic causes; The causes of IBS aren’t fully understood. such factors as the impairment of gastrointestinal motility or autonomic nerves system, altered gastrointestinal sensitivity or gut microbiota, stress and genetic are mentioned. Although the development of IBS is reported after viral gastroenteritis, the association of IBS with viral infection is not clear. Since Adenoviruses are common causes of human gastroenteritis, we decided to investigate the association of IBS with Adenoviruses.

80 subjects (40 patients with IBS and 40 healthy subjects) were recruited and studied after obtaining informed consent. In all subjects, a blood sample was taken for determination of Adenoviruses antibody IgG and IgM (Elisa-method), as well as a stool sample for adenovirus DNA. Viruses DNA was extracted with phenol-chloroform-isoamyl alcohol and evaluated with Real-time PCR based on SYBR-Green.

According to data analysis, there was no significant difference between IBS group and control group. Adenoviruses antibody IgG, IgM and DNA load didn’t show any significant raise in IBS group.

We didn’t observe any significant association between IBS and Adenoviruses. However, additional studies are recommended to investigate the role of other viruses in development of IBS.