Irritable bowel syndrome (IBS) is a common functional gastrointestinal disorder linked to gut microbiota alterations. This study aimed to evaluate the abundance of crAssphage in IBS patients compared to healthy controls using quantitative PCR (qPCR).

The study comprised 38 patients with irritable bowel syndrome (IBS) and 40 healthy control subjects. All participants underwent clinical and laboratory assessments. Stool samples were collected from both IBS patients and healthy controls, and the abundance of crAssphage was determined using quantitative PCR (qPCR).

Quantitative PCR analysis showed that the abundance of crAssphage was lower in patients with irritable bowel syndrome (IBS) compared to healthy control subjects; however, the difference was not statistically significant (p > 0.05).

Although the abundance of crAssphage tended to be lower in IBS patients, the difference was not statistically significant. These findings suggest that crAssphage may be affected in IBS, but further studies with larger sample sizes are needed to clarify its potential role in gut microbial alterations associated with the disorder.