This study presents an innovative and efficient tumor-targeted nanotherapeutic using superparamagnetic nanoparticles (SPIONs) conjugated with anti-EGFR Panitumumab. We conducted comprehensive characterizations and assessments of this nanotherapeutic to evaluate its biological effects and photothermal properties in SW-480 colorectal cancer cells. In order to assess the efficacy of this nanbiosystem in combination with photothermal therapy .(PTT), we employed low-intensity near-infrared (NIR) laser irradiation.

Targeted SPION-Pan nanomedicine was synthesized, characterized, and evaluated for cytotoxic assay and apoptosis induction on KRAS mutant SW-480 colorectal cancer cells combined with photothermal therapy (PTT) using near-infrared (NIR) laser irradiation.

The study findings demonstrated that the targeted nanobiosystem resulted in diminished cell viability and the induction of apoptosis in the SW480 cell line. Furthermore, our investigation showed that the combination therapy (PTT + SPION-Pan) had a synergistic effect on promoting cancer cell apoptosis. Notably, after a 2-hour and 24-hour exposure to low-intensity NIR irradiation at 0.5 milliwatts, the IC50 value of SPION-Pan significantly decreased when compared to cells treated with SPION-Pan alone, without NIR laser irradiation, or NIR laser irradiation alone.

We introduced a potent nanoformulation of panitumumab in combination therapy with PTT, demonstrating a remarkable capacity to overcome resistance to anti-EGFR monoclonal antibodies and induce apoptosis in colorectal cancer cells, outperforming the efficacy of free antibodies