Sirtuins, known as the intracellular acylation enzymes, play a major role in regulating the cell's physiological activities. The relevant studies have shown diversely sirtuin genés expression in human֝s various cancers. This study has surveyed the transcription of sirt3, 6, and 7 genes in gastric antral epithelial cells of gastritis and gastric adenocarcinoma patients with and without H. pylori infection
First of all, a case-control study was conducted, including 50 and 53 gastric antral biopsy samples collected from gastritis and gastric adenocarcinoma patients with and without H. pylori infection referred to hospitals of Sanandaj city during 2018-2019. Total RNAs were extracted from biopsy samples, then cDNAs were synthesized by using Takara kits. Quality essay of H. pylori virulence genés expression and relative quantitative essay of sirt3, 6, and 7 genés expressions in gastric antral biopsy samples were performed using the Real-Time RT PCR method.
The statistical analysis showed the significant correlations between H. pylori vacA s1m2 and sabA cDNAs with sirt3 geneś expression in gastric antral epithelial cells [p<0.05, 0.05 respectively]. In addition, sirt6 gene's expression decreased along increasing age in gastric adenocarcinoma patients [p<0.05]. The samples of gastritis patients֝ gastric antral epithelial biopsy containing H. pylori hopQII, oipA, and sabB cDNA showed an increased amount of sirt7 geneś expression [p<0.05, 0.05, and 0.05 respectively].
The H. pylori virulence genes֝ expression and increasing age of patients showed the significant correlations with sirt3, 6, and 7 geneś expressions in gastric antral epithelial cells of gastric and gastric cancer patients.