Hepatitis b DNA polymerase chain reaction (HBV DNA PCR) is a test that used in evaluation and treatment of hepatitis b but this test is pretty expensive and may be not available everywhere Serum hepatitis B surface antigen quantitation test may be surrogate test that was available and much low price Compare the accuracy of two test

HBsAg positive Patients that referred to lab Boali in Yazd - Iran for HBV DNA test in 2012 -2014 were selected and divided into three groups for initial diagnosis inactive carrier, beginning of treatment and on treatment, thirty in each group . (HBV DNA PCR were performed with real time PCR method with COBAS Taq Man HBV test (Roche Diagnostics) with sensivity of 150 IU/ml. HBsAg and HBeAg level is measured by Electochemiolance LIASION device .level of upper than 0.05/ml was consider positive for HBsAg.

Serum hepatitis B surface antigen quantitve level was significantly different between group one and group two also between group one and group three (p=0.001) but not different between group two and three (p=0.7) Serm HBV Quntitive level and HBV DNA was a postive relation (P=0.001,R=0.527)There is relation between HBsAg and viral load in group one (P=0.017, R=0.431) and group two (P=0.023, R=0.427 )but don’t correlated in group three (P=0.27, R=0.22) .

HBsAg quantititive measurement may be a surrogate test for evaluation of hepatis b patients that more simple and economic