Colorectal cancer (CRC) as a major health problem has been increased globally. Etiology of CRC is one of critical issues. Smoking, obesity and infectious disease are such probable risk factors of CRC. Meanwhile, chronic infection such as chronic hepatitis B (CHB) is under investigation. The aim of present study was to determine the rate of HBV genome infection in CRC patients compared to healthy subject’s colon tissue.

Archived formalin-fixed paraffin-embedded (FFPE) blocks of 157 patient underwent total colonoscopy that were referred to hospitals affiliated to Iran University of Medical Sciences, Tehran, IR Iran, enrolled. They categorized into 66 CRC cases and healthy colon tissue as control group. After DNA extraction from FFPE specimens a Syber Green Real-time PCR method carried out. SPSS v16 used for statistical analysis.

from a total of 157 specimens, 66 cases mean age ± Std. deviation was 59.3 ± 14.4 and 57.6% (38/66) of them were males. Of 91 healthy controls mean age ± Std. deviation was 57.2 ± 14.6 and 57.1% (52) of them were males. By Real-time PCR we found there were 6.4% (10/157) HBV positive that of them 9% (6/66) were CRC and 4.4% (4/91) were healthy controls. Different variables had not any significant results.

although some studies reported the association of HBV infection in CRC outcome, we did not find any correlation that suggest the role of interfering other risk factors. Further studies by broader sample size and different study population recommended.